Evaluation of modified petroff’s method and manual MGIT culture system to facilitate detection of acid-fast bacilli in smear-negative presumptive cases of pulmonary tuberculosis
DOI:
https://doi.org/10.47203/IJCH.2019.v31i02.013Keywords:
Modified Petroff; S method; Concentrated smear technique; MGIT; TuberculosisAbstract
Background: Tuberculosis is a major public health problem in India. According to RNTCP guidelines, all efforts are to be made to diagnose tuberculosis on the basis of detection of AFB/ MTB in the clinical specimen; hence all those cases where AFB/ MTB have not been detected are presumptive cases of tuberculosis. In this background, the present study aims to detect AFB/ MTB in the sputum of clinico-radiologically presumptive cases of pulmonary tuberculosis using direct ZN microscopy, Modified Petroff’s method followed by ZN microscopy & MGIT liquid culture method. Materials and Methods: A total of 94 sputum samples which were negative for AFB by direct ZN microscopy at the DMC of the institute, were subjected to Modified Petroff’s method followed by microscopy & MGIT liquid culture test to find out any additional yield of bacteriologically confirmed disease. Result: Out of 94 specimens, 20 (21.28%) were positive for AFB on ZN microscopy post Modified Petroff’s method. Among all the specimens, 30 (31.91%) were found to be positive by MGIT liquid culture method with average time to detection growth (TTD) around 16.73 days (2-38 days). Conclusion: Modified Petroff’s method followed by ZN microscopy & MGIT test for mycobacterial culture can improve case detection.
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References
Zaman K. Tuberculosis: A global health problem. J Health PopulNutr 2010; 28(2):111-3.
Behr MA, Warren SA, Salamon H, Hopewell PC, Ponce de Leon A, Daley CL. Transmission of mycobacterium tuberculosis from patients smear negative for acid-fast bacilli. Lancet 1999; 353:444-9.
Marei AM, El-Behedy EM, Mohtady HA, Afify AF. Evaluation of a rapid bacteriophage-based method for the detection of Mycobacte¬rium tuberculosis in clinical samples.J Med Microbiol. 2003;52:331-335
McNerney R. Diagnosis: present difficulties and prospects for the future. Afr Health 1996; 19:22-23.
Nancy DE, John B, Paula F, Philip H, Robert CH, Max S, Patricia MS. Diagnostics standards and classification of tuberculosis in adults and children. Am J Resp. Crit Care Med 2000; 1611376-1395.
Badak FZ, Kiska DL, Setterquist S, Hartley C, O’Connell MA, Hop¬fer RL. Comparison of Mycobacteria Growth Indicator Tube with BACTEC 460 for detection and recovery of mycobacteria from clinical specimens. J ClinMicrobiol.1996; 34:2236-2239.
Mistry Y, Rajdev S, Mullan S, Use of Cost Effective Semi-Automated(Mannual/Micro) MGIT System over BACTEC 960to Perform First Line Anti-Tuberculosis DrugsSensitivityTesting.J of TB Res 2016; 4:227-234.
Rishi, S., Sinha, P, Malhotra, B. and Pal, N. A Comparative Study for the Detection of Mycobacteria by BACTEC MGIT 960, Lowenstein Jensen Media and Direct AFB Smear Examination. Indian J of Med Microbiol. 2007; 25:383-386.
Rawat J,Biswas D, Sindhwani G, VictorMasih V and Chauhan BS. Diagnostic role of MGIT culture of bal samples in sputum smearnegative pulmonary tuberculosis. Ind.J.of Tuberculosis.2013; 60:77-82.
Rodrigues C, Shenai S, Sadani M, Sukhadia N, Jani M, Ajbani K, Sodha A, Mehta A, Evaluation of the BactecMgit 960TBsystem for recoveryand identification of Mycobacterium Tuberculosis complex in a high through put Tertiary Care Centre. Ind J of Med Micro. 2009; 27;3:217-21
Rodrigues CS, Shenai SV, Almeida D, Sadani MA, Goyal N, Vadher C, Mehta AP.Use of BACTEC 460 TB system in the diagnosis of TUBERCULOSIS. Ind.J.of Med Micro. 2007; 251:32-6.
Fadzilah MN, KeePeng NG, Fong NY. The manual MGIT system for the detection of M tuberculosis in respiratory specimens: an experience in the University Malaya Medical Centre. Malaysian J Pathol. 2009; 31(2):93 – 97
Uddin MN, Uddin MJ, Mondol MEA, Islam SMJ, Wadul VBM. Comparison of conventional and automated culture system for isolation of Mycobacterium Tuberculosis. JAFMC. 2009; 5:1:14-17.
Chakrabartty AK, Ali KM, Ghosh A, Ghosh D, A Study On Exclusion Group From The Initiation Of Dots Under Revised National Tuberculosis Control Programme In West Bengal, India Int J Cur Res 2015; l 7:6:24-32
Mashruk et al. studies on acquired drug resistance pattern of M.tuberculosis (Thesis) Dhaka : BSMMU; 2000.p. 80.
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