Evaluation of modified petroff’s method and manual MGIT culture system to facilitate detection of acid-fast bacilli in smear-negative presumptive cases of pulmonary tuberculosis

  • Sana Jamali Integral Institute of Medical Sciences & Research, Lucknow, Uttar Pradesh
  • Richa . Dr SN Medical College, Jodhpur, Rajasthan
  • Vinod K Srivastava Integral Institute of Medical Sciences Research, Lucknow, Uttar Pradesh
Keywords: Modified Petroff; S method; Concentrated smear technique; MGIT; Tuberculosis

Abstract

Background: Tuberculosis is a major public health problem in India. According to RNTCP guidelines, all efforts are to be made to diagnose tuberculosis on the basis of detection of AFB/ MTB in the clinical specimen; hence all those cases where AFB/ MTB have not been detected are presumptive cases of tuberculosis. In this background, the present study aims to detect AFB/ MTB in the sputum of clinico-radiologically presumptive cases of pulmonary tuberculosis using direct ZN microscopy, Modified Petroff’s method followed by ZN microscopy & MGIT liquid culture method. Materials and Methods: A total of 94 sputum samples which were negative for AFB by direct ZN microscopy at the DMC of the institute, were subjected to Modified Petroff’s method followed by microscopy & MGIT liquid culture test to find out any additional yield of bacteriologically confirmed disease. Result: Out of 94 specimens, 20 (21.28%) were positive for AFB on ZN microscopy post Modified Petroff’s method. Among all the specimens, 30 (31.91%) were found to be positive by MGIT liquid culture method with average time to detection growth (TTD) around 16.73 days (2-38 days). Conclusion: Modified Petroff’s method followed by ZN microscopy & MGIT test for mycobacterial culture can improve case detection.

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Published
2019-06-30
How to Cite
1.
Jamali S, . R, Srivastava VK. Evaluation of modified petroff’s method and manual MGIT culture system to facilitate detection of acid-fast bacilli in smear-negative presumptive cases of pulmonary tuberculosis. Indian J Community Health [Internet]. 2019Jun.30 [cited 2020Aug.5];31(2):231 -235. Available from: https://www.iapsmupuk.org/journal/index.php/IJCH/article/view/1098
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Original Article